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After 24 hours the media was replaced and unattached cells removed. All experiments were conducted with triple or quadruple values at each point and all plates contained positive glutamate alone and baseline controls. Examples of useful substituted alkoxy groups are: As shown in FIG. Unlike cannabidiol, THC is a ligand and agonist for the brain cannabinoid receptor. T-butyl hydroperoxide, tetraethylammonium chloride, ferric citrate and sodium dithionite were all purchased from Aldrich WI. Special attention was used to ensure the solution did not overheat or generate foam. Astrocyte cultures were used to condition DMEM for no longer than two months. The voltage at which the first peak occurs is an indication of the voltage at which an electron is donated, which in turn is an index of antioxidant activity.


Fetuses were extracted by Cesarian section from a 17 day pregnant Wistar rat, and the feral brains were placed into phosphate buffered saline. In after reviewing all evidence brought forth in a lawsuit against the government's prohibition of medical marijuana--the DEA's own administrative law judge Judge Francis Young wrote: Brains were then fixed for 24 hours in formaldehyde, sliced and the infarct volumes measured. The infarct size and neurological assessment of the test animals is shown Table 1. Several other natural and synthetic cannabinoids, including cannabidiol, nabilone, and levanantrodol were also tested, and they too exhibited oxidation profiles similar to cannabidiol and THC data not shown. Primary neuronal cultures were prepared according to the method of Ventra et al. These and other objects of the invention will be understood more clearly by reference to the following detailed description and drawings. T-butyl hydroperoxide, tetraethylammonium chloride, ferric citrate and sodium dithionite were all purchased from Aldrich WI. In particular, ROS are believed to accumulate when tissues are subjected to ischemia, particularly when followed by reperfusion. Tetraethylammonium chloride in acetonitrile 0. HetAr is a heteroaryl group, and R is a straight-chain or branched chain aliphatic group. Oxidative associated diseases include, without limitation, free radical associated diseases, such as ischemia, ischemic reperfusion injury, inflammatory diseases, systemic lupus erythematosis, myocardial ischemia or infarction, cerebrovascular accidents such as a thromboembolic or hemorrhagic stroke that can lead to ischemia or an infarct in the brain, operative ischemia, traumatic hemorrhage for example a hypovolemic stroke that can lead to CNS hypoxia or anoxia , spinal cord trauma, Down's syndrome, Crohn's disease, autoimmune diseases e. Patent The U. Cell toxicity was assessed hours after insult by measuring lactate dehydrogenase LDH release into the culture media. Assays for selecting compounds with the desired effect on lipoxygenase enzymes, and methods for using identified compounds to treat neurological or ischemic diseases are also provided. Anandamide, a cannabinoid receptor ligand without a cannabinoid like structure, was used as a non-responsive control. Specific AMPA and kainate receptor ligands were also used to separately examine the effects of cannabinoids on AMPA and kainate receptor mediated events. The invention also includes an assay for selecting a cannabinoid to use in treating a neurological disease by determining whether the cannabinoid is an antioxidant. Nonpsychoactive cannabinoids, such as cannabidoil, are particularly advantageous to use because they avoid toxicity that is encountered with psychoactive cannabinoids at high doses useful in the method of the present invention. The cannabinoids are found to have particular application as neuroprotectants, for example in limiting neurological damage following ischemic insults, such as stroke and trauma, or in the treatment of neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease and HIV dementia. The invention also includes identifying and administering antioxidant and neuroprotective compounds such as cannabidiol which selectively inhibit the enzyme activity of both 5- and lipoxygenase more than the enzyme activity of lipoxygenase. Examples of useful substituted alkoxy groups are: However the present invention can also be used as an antioxidant treatment in non-neurological diseases. The level of lactate dehydrogenase LDH in the media was used as an index of cell injury. In an unsubstituted alkoxy, the R is an unsubstituted alkyl. The hydroxyl radical sets off chain reactions and can interact with nucleic acids.


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21.09.2018 at 10:12 pm

Such diseases may include a vascular ischemic event in the central nervous system, for example a thromboembolism in the brain, or a vascular ischemic event in the myocardium. All living tissues were stained pink by the dye, while morbid regions of infarcted tissue remained white.

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